质粒dna提取的时候,为什么要加氨苄青霉素

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质粒dna提取的时候,为什么要加氨苄青霉素

质粒dna提取的时候,为什么要加氨苄青霉素
质粒dna提取的时候,为什么要加氨苄青霉素

质粒dna提取的时候,为什么要加氨苄青霉素
汗,没听说过氨苄青霉素可以破坏细胞壁的,这样子的话就不需要碱裂解了,氨苄青霉素肯定是在扩增菌体的时候加入培养基的,不会对提取本身造成影响,但是可以阻止杂菌生长,增加目的质粒的拷贝数(前提是,质粒是氨苄抗性的,别加错啦)

Your plasmid must be carrying ampicillin-resistant gene. Therefore, you should include ampicillin when you are growing your culture for plasmid preparation. It guarantees the plasmid present in the bacterial cells in your culture, and hence increases the plasmid DNA yield.
Hope that is what you are asking.

2楼说的非常好